Fig. 1.

Antidepressant-like effects in the forced swim test after ACD855 administration. The forced swim test was conducted using a 10 min pre-test (day 1) followed 24 h later (day 2) by a 6 min test session where the immobility was recorded. Mice were injected either with a single dose of ACD855 (3 mg/kg, s.c.; panel a) on day 2 prior to behavioral test or repeated administration (once daily; 3 mg/kg/day, s.c.) for 4 or 28 consecutive days (panel b and c respectively) before behavioral testing, and were compared with mice receiving vehicle or mice treated with a single dose of 20 mg/kg fluoxetine on day 2 prior to behavioral test. Both fluoxetine and ACD855 (p < 0.01; p < 0.001; p < 0.0001) significantly reduced the immobility time in the forced swim test compared with vehicle treated mice. The bars represent the immobility time (seconds), mean ± SEM (n = 6–8 mice per group). The statistical analysis was performed using one-way ANOVA followed by Tukey's test (panel A and B), or unpaired t-test (C). **p < 0.01; ***p < 0.001; ****p < 0.0001 vs control group