Fig. 5.

Antidepressant-like effects of different doses of fluoxetine in the forced swim test. The forced swim test was conducted using a 10 min pre-test (day 1) followed 24 h later (day 2) by a 6 min test session where the immobility was recorded. Mice were injected day 2 prior to behavioral test either with acute single doses of fluoxetine (5, 10 and 20 mg/kg, i.p.) or administered both fluoxetine (10 mg/kg, i.p.) and AC-0026845 (0.1 mg/kg, s.c.) and were compared with vehicle treated mice or mice given a single dose of fluoxetine (10 mg/kg) or AC-0026845 (0.1 mg/kg). Fluoxetine at doses 5, 10 and 20 mg/kg induced a significant decrease in immobility time at 10 and 20 mg/kg (Fig. 5a, p < 0.05; p < 0.0001, respectively) compared with control mice. Administration of a subthreshold dose of AC-0026845 (0.1 mg/kg) in combination with fluoxetine (10 mg/kg) induced a significant decrease in immobility time as compared to control, AC-0026845 (0.1 mg/kg) or fluoxetine (10 mg/kg) alone (Fig. 5b, p < 0.01, p < 0.05; p < 0.05, respectively). The bars represent the immobility time (seconds), mean ± SEM (n = 6 mice per group). The statistical analysis was performed using one-way ANOVA (Fig. 5a) and two-way ANOVA (Fig. 5b) respectively, followed by Tukey's test. *p < 0.05; ****p < 0.001vs control; #p < 0.05 vs fluoxetine treated mice; §p < 0.05vs AC-0026845 treated mice