Fig. 6.

Antidepressant-like effects of different doses of Ketamine in the forced swim test. The forced swim test was conducted using a 10 min pre-test (day 1) followed 24 h later (day 2) by a 6 min test session where the immobility was recorded. Mice were injected day 2 prior to behavioral test either with acute single doses of ketamine (2.5, 5, and 10 mg/kg, i.p.) or administered both a sub-threshold dose of ketamine (5 mg/kg, i.p.) and AC-0026845 (0.1 mg/kg, s.c.) and were compared with vehicle treated mice and mice treated with a single dose of ketamine (5 mg/kg) or AC-0026845 (0.1 mg/kg). Ketamine at doses 2.5, 5, and 10 mg/kg induced a significant decrease in immobility time at 10 mg/kg (Fig. 6a, p < 0.01) compared with control mice. Administration of AC-0026845 (0.1 mg/kg) in combination with ketamine (5 mg/kg) induced a significant decrease in immobility time as compared to control and AC-0026845 treated mice (Fig. 6b, P < 0.05; p < 0.05, respectively). The bars represent the immobility time (seconds), mean ± SEM (n = 7–8 mice per group). The statistical analysis was performed using one-way ANOVA (Fig. 6a) and two-way ANOVA (Fig. 6b) respectively, followed by Tukey's test. *p < 0.05 vs control; #p < 0.05 vs AC-0026845 treated mice