Table 8.
Primer sequences and PCR conditions for genotyping of HLA-I ligands by PCR-SSP method.
| Primer sequences (5ʹ─3ʹ) | PCR conditions | Amplicon size | Ref | ||
|---|---|---|---|---|---|
| 1 | HLA-A*03 |
F: CACGGAATGTGAAGGCCCAG R: CGTGGGGGATGAGGGGTCC |
95 °C/3 min 10 cycles: 94 °C/20 s, 72 °C-62°C/30 s (− 1 °C per cycle) and 72 °C/30 s 20 cycles: 94 °C/20 s, 62 °C/30 s and 72 °C/30 s 72 °C/5 min |
129 | New |
| 2 | HLA-A*11 |
F: CACGGACGGGCCAGGTG R: CCCTCCAGGTAGGCTCTCT |
95 °C/3 min 9 cycles: 94 °C/20 s, 72 °C-63°C/30 s (− 1 °C per cycle) and 72 °C/30 s 21 cycles: 94 °C/20 s, 63 °C/30 s and 72 °C/30 s 72 °C/5 min |
410 | New |
| 3 | HLA-A*23/24 |
F: GGTCTCAGCCACTCCTCGT R: GCTCTGGTTGTAGTAGCGGA |
95 °C/3 min 12 cycles: 94 °C/20 s, 72 °C-60°C/30 s (− 1 °C per cycle) and 72 °C/30 s 18 cycles: 94 °C/20 s, 60 °C/30 s and 72 °C/30 s 72 °C/5 min |
288 | New/Tajik48 |
| 4 | HLA-A*32 |
F: GGTGAGTGCGGGGTCGT R: GCTCTGGTTGTAGTAGCGGA |
393 | New/Tajik48 | |
| 5 | HLA-B Bw6 |
F: GAGCGAGGGGACCGCAG R: TGTAGTAGCCGCGCAGGT |
95 °C/3 min 8 cycles: 94 °C/20 s, 72 °C-64°C/30 s (− 1 °C per cycle) and 72 °C/30 s 22 cycles: 94 °C/20 s, 64 °C/30 s and 72 °C/30 s 72 °C/5 min |
344 | Tajik48/Hong49 |
F: forward primer, R: reverse primer.