Table 2.

Physicochemical properties, instability index and binding energy of the Kunitz4 derived peptides.

Peptide name	Binding energy (kcal/mol)	GRAVYa	Net charge at PH 7	Water solubility	Instability indexb	Isoelectric point	HPLC purity (%)	Salt form
Kunitz4-a	− 770.7	− 0.900	2	Good	117.23	11.21	95.28	Acetate
Kunitz4-b	− 1673.8	− 1.283	0.9	Good	35.83	8.77	96.31	Acetate
Kunitz4-c	− 1488.5	− 2.046	5.1	Good	80.85	11.9	95.28	Acetate

^aPositive GRAVY values indicate hydrophobic; negative values mean hydrophilic.

^bInstability index value < 40 indicates that the peptide is stable.