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. 2023 Jul 15;14:4233. doi: 10.1038/s41467-023-39961-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Domain organization and b multiple sequence alignment of human RBM5, RBM6, and RBM10 proteins. RNP2 and RNP1 motifs of RRM1 and RRM2 are boxed in orange and domain boundaries of RRM1, ZnF1, and RRM2 are highlighted in blue, green, and pink, respectively. Linkers L0 and L1 connecting RRM1-ZnF1 and ZnF1-RRM2, respectively are highlighted in gray. The non-canonical hydrophobic residues found instead of the canonical aromatic residues in RNP2 motif of both RBM5 RRM1 and RRM2 domains are colored in red. c Overlay of ¹H-¹⁵N HSQC spectra of three RRM1-ZnF1_S-RRM2 domains construct (black) and single RRM1 (light blue) and RRM2 (purple) domains. d Overlay of ¹H-¹⁵N-HSQC spectra of the three RRM1-ZnF1_S-RRM2 domains construct (black) with that of RRM1-ZnF1_S-L1 (orange) and RRM1-ZnF1_S (green). Zoomed representative residues are shown. e Tumbling correlation time τ_c values (calculated from the ratio of ¹⁵N R₂/R₁ relaxation rates) are plotted vs. residue number for RRM1-ZnF1_S-RRM2 (black), RRM1-ZnF1_S-L1 (orange) and RRM1-ZnF1_S (green) and their domain-wise average τ_c values ± SD are indicated in f. Error bars are derived from relaxation data. Source data are provided as a Source Data file.