Table 3.

Isothermal titration calorimetry data for RBM5 RNA binding domains

Protein	RNA	Av. KDa (μM)	KDb (μM)	ΔHb (kcal/mol)	−TΔSb (kcal/mol)	Binding stoichio-metry (N)b
RRM1	CU_9	19.7 ± 6.5	13.2 ± 3.1 26.2 ± 6.4	−7.3 ± 0.8 −10.0 ± 1.7	0.61 3.76	1.04 1 (fixed)
RRM1	GGCU_12	1.88 ± 0.95	0.93 ± 0.18 2.83 ± 0.47	−11.7 ± 0.4 −10.6 ± 0.4	3.49 3.06	0.97 1.01
RRM2	GGCU_12	172 ± 6	171 ± 8 174 ± 8	−24.5 ± 0.7 −25.8 ± 0.8	19.2 20.6	1 (fixed) 1 (fixed)
RRM1-ZnF1 wt	CU_9	5.56 ± 2.83	8.39 ± 0.87 2.73 ± 0.37	−11.8 ± 1.1 −6.23 ± 0.27	4.88 1.36	1.02 0.94
RRM1-ZnF1 wt	GGCU_12	0.153 ± 0.069	0.084 ± 0.012 0.222 ± 0.040	−16.2 ± 0.2 −14.1 ± 0.3	6.57 4.99	1.02 0.81
RRM1-ZnF1 F142/144A	GGCU_12	0.448 ± 0.036	0.469 ± 0.055 0.427 ± 0.047	−12.7 ± 0.2 −12.0 ± 0.2	4.05 3.31	0.7 0.97
RRM1-ZnF1 F202A	GGCU_12	0.379 ± 0.041	0.368 ± 0.046 0.390 ± 0.067	−9.58 ± 0.20 −9.74 ± 0.27	0.80 1.00	1.03 1.01
RRM1-ZnF1 R198E	GGCU_12	2.66 ± 0.38	2.69 ± 0.27 2.63 ± 0.70	−10.7 ± 0.3 −8.37 ± 0.83	3.08 0.75	1.05 1.11
RRM1-ZnF1S	GGCU_12	0.263 ± 0.027	0.273 ± 0.036 0.253 ± 0.041	−10.5 ± 0.2 −10.9 ± 0.2	1.56 1.94	1.08 1.06
RRM1-ZnF1S	eGGCU_12	0.641 ± 0.125	0.766 ± 0.116 0.516 ± 0.077	−15.2 ± 0.4 −14.2 ± 0.3	6.90 5.66	0.63 0.60
RRM1-ZnF1S-RRM2	GGCU_12	0.133 ± 0.012	0.127 ± 0.007 0.138 ± 0.022	−19.1 ± 0.1 −18.1 ± 0.4	9.68 8.70	0.98 1.01

^aK_D values determined from duplicate measurements, with standard deviations as indicated.

^bValues obtained for individual measurements are listed.