Table 1.
Composition of media used in mono- and co-culture experiments and MTT assay.
| Code Name (Fig. 4) | M1 | M2 | M3 |
|---|---|---|---|
| Abbreviation | KGM2 | DMEM | 50% KGM2 + 50% DMEM |
| Additives | - Bovine pituitary extract | (50%) | |
| - Epidermal growth factor | - Bovine pituitary extract | ||
| - Insulin | - Epidermal growth factor | ||
| - Hydrocortisone | + 10% fetal bovine serum | - Insulin | |
| - Epinephrine | + Neomycin (20 µg ml−1) | - Hydrocortisone | |
| - Transferrin | + Kanamycin (100 µg ml−1) | - Epinephrine | |
| - CaCl2 | - Transferrin | ||
| - Neomycin (20 µg ml−1) | - CaCl2 | ||
| - Kanamycin (100 µg ml−1) | (50%) | ||
| + 10% fetal bovine serum | |||
| (100%) | |||
| ± Neomycin (20 µg ml−1) | |||
| ± Kanamycin (100 µg ml−1) | |||
| Cell Type | Keratinocytes | Fibroblasts | Keratinocytes + Fibroblasts |