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. 2023 Jul 15;14(7):435. doi: 10.1038/s41419-023-05968-w

Fig. 6. FAO is required to maintain genomic stability.

Fig. 6

A Cell viability of immortalized MEFs treated with the indicated drugs. Cells were treated with or without 0.05 μM DOX, 200 μM ETO, 0.5 mM citrate, and/or 50 μM octanoate for 48 h. Statistical analysis was based on one-way ANOVA with Tukey’s multiple comparisons test. B Cell viability of the 293 T cells, reconstituted with WT or two mutant forms of PARP1, treated with DOX. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test. C Immortalized MEFs were treated with or without 10 μM ETS, 200 μM ETO, 0.1 mM citrate, and/or 50 μM octanoate for 15 h. The following day, growth media was replaced with fresh media. Two days later, cells were stained with propidium iodide and analyzed by flow cytometry. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. D Percentages of multinucleated, binucleated, and mononucleated cells in the indicated cells. Immortalized MEFs were treated with ETS, ETO, citrate and/or octanoate as indicated. The number of cells pooled from three independent experiments is indicated. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test. The indicated p-values represent comparison within multinuclei. Representative images of cells staining with DAPI and anti-phalloidin antibody as indicated (right). Scale bar represents 20 μm. E A proposed model for role of FAO in DNA repair. All error bars ± SEM. **p < 0.01, ***p < 0.001 and ****p < 0.0001.