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[Preprint]. 2023 Jul 3:2023.07.03.547547. [Version 1] doi: 10.1101/2023.07.03.547547

Fig. 1 |. β-hydroxybutyrate directly induces protein insolubility without posttranslational modification.

Fig. 1 |

a, Schematic of experimental procedures. b-c, Imperial staining and quantification of soluble and induced-insoluble native (+37°C) and heat-misfolded (+70°C) BSA treated with (b) 10 mM of R-βHB or 10 mM of alcohol analogue, 1,3-BD, and (c) 5–10 mM of R-βHB or 1–2 mM of nonenzymatic elicitor of KβHB, (R)-βHB-SNAC. d, Imperial staining and quantification of 24 month male wild-type mouse soluble cytosolic brain proteins which remain soluble or are insolubilized after treatment with 10 mM of a small library of 15 metabolites.

b, Mean ± S.E.M, N=3, p-value calculated using one-way ANOVA with Sidak’s multiple comparison test.

c, Mean ± S.E.M, N=3, p-value calculated using one-way ANOVA with Sidak’s multiple comparison test.

d, Mean ± S.E.M, N=3, p-value calculated using one-way ANOVA with Dunnett’s multiple comparison test.