Extended Data Fig. 3 |. β-hydroxybutyrate remodels the older C57BL/6 mouse brain proteome ex vivo via insolubilization of targets.

a, Schematic of ex vivo protein insolubilization assay. b, Imperial staining and quantification of 24 month female wild-type mouse soluble cytosolic brain proteins which remain soluble or are insolubilized after treatment with 1–10 mM of R-βHB and 1–10 mM of 1,3-BD. c-d, Dotplots from clusterProfiler gene ontology overrepresentation analysis for biological process on top 10 significantly upregulated terms in (c) 1/0 mM and (d) 5/0 mM R-βHB treatment groups.

b, Mean ± S.E.M, N=3, p-value calculated using one-way ANOVA with Dunnett’s multiple comparison test.