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[Preprint]. 2023 Jul 5:2023.07.04.547745. [Version 1] doi: 10.1101/2023.07.04.547745

Figure 1. VPS50 gene edition causes decrease in synaptic vesicle acidification but no change in synaptic vesicle number.

Figure 1.

(A) Surveyor assay of Control or VPS50 mKO cortical neurons. (B) RT-qPCR to quantify relative mRNA expression of VPS50 mKO. (C-D) VPS50 protein expression in VPS50 mKO and Control neurons. (E-F) Representative images of synaptic terminals by electron microscopy and quantification of the number of synaptic vesicles in Control and VPS50 mKO neurons (n=50 cells per condition). (G) Representative images of Ratio-SyPhy signal to determine synaptic vesicle acidification in Control and VPS50 mKO neurons. Scale bar, 25um. ***p<0.001.