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[Preprint]. 2023 Jul 5:2023.07.03.547455. [Version 2] doi: 10.1101/2023.07.03.547455

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Figure 2. — (A,B) Overall vascular patterning of she−/− mutants is normal when compared to their sibling wild-type embryos at 28 hpf. Embryos are in kdrl:GFP background.

(C-H) A wider DA is observed in she mutant embryos compared to their wild-type (she+/+) siblings at 1 and 2 dpf (28 and 48 hpf respectively). Red line indicates DA diameter. (G,H) shows higher magnification image of trunk vasculature at 1 dpf.

(I,J) DA is narrower in she mutants at 4 dpf compared to their siblings.

(K) Diameter of the DA and PCV at 1–4 dpf in she mutants and their wild-type siblings. * p<0.05; ***p<0.001, NS – not significant, Student t-test. Error bars show SEM.

At all stages, she mutant and sibling embryos were obtained by in-crossing she^ci26+/−; kdrl:GFP carriers. Embryos at 1 and 2 dpf were genotyped after imaging. Embryos at 4 dpf were separated based on the phenotype, and wild-type siblings include she+/+ and she+/− embryos at this stage.