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[Preprint]. 2023 Jul 5:2023.07.03.547455. [Version 2] doi: 10.1101/2023.07.03.547455

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Figure 4. — (A,B) Pericardial edema is observed in she mutant embryos at 4 dpf (A), while embryos positive for hsp70:she-2A-mCherry do not show pericardial edema and have normal blood circulation after heat-shock was performed at 3 dpf (B). Embryos were obtained from the cross of hsp70:she-2A-mCherry; cryaa:dsRed +/−; she+/− X she+/− parents in kdrl:GFP background. Embryos were sorted for dsRed in the lens (driven by the lens specific cryaa promoter which correlates with the presence of hsp70:she-2A-mCherry transgene) and mCherry in the body. Representative embryos with and without edema are shown for each group.

(C-F) Dorsal aorta in she mutants is narrower or collapsed at 4 dpf, while heat-shock at 3 dpf restores normal DA size in she−/−; hsp70:she-2A-mCherry embryos.

(G) Percentage of total embryos showing the pericardial edema phenotype at 4 dpf. Embryos were obtained by crossing hsp70:she-2A-mCherry; cryaa:dsRed +/−; she+/− X she+/− adults in kdrl:GFP background and subjected to heat-shock (HS) at 3 or 3.5 dpf. Embryos with dsRed in the lens (Tg+) or transgene negative controls (Tg-) were selected for the analysis. Tg- embryos were also subjected to heat shock at 3 or 3.5 dpf (both groups were combined).

(H) The diameter of the dorsal aorta at 4 dpf in wild-type siblings (includes heterozygous and wild-type embryos), she mutant embryos without the transgene, and she−/−; hsp70:she-2A-mCherry embryos heat-shocked at 3 or 3.5 dpf. ** p<0.01, Student’s t-test.