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[Preprint]. 2023 Jul 5:2023.07.03.547455. [Version 2] doi: 10.1101/2023.07.03.547455

Figure 5. she affects cell number and inhibits endothelial cell proliferation.

Figure 5.

(A-C) Analysis of cell number in the DA of she mutant and wild-type (she+/+) sibling embryos at 72 hpf. Embryos were obtained from an incross of she+/−; fli1:GFP; kdrl:NLS-mCherry parents, imaged by confocal microscopy and subsequently genotyped. Note increased cell number in she mutant embryos. Data combined from 2 independent experiments. ***p<0.001, Student’s t-test. (D-F) Analysis of cell number in the DA of fli1:she-2A-mCherry; fli1:NLS-GFP embryos and their mCherry negative (wt) siblings at 72 hpf. Note the reduced cell number in mCherry+ embryos. Data combined from 2 independent experiments. ****p<0.0001, Student’s t-test. (G-M) Cell proliferation analysis using BrdU incorporation assay in she−/−; fli1:she-2A-mCherry embryos (phenotypically normal) and their sibling mCherry negative she−/− embryos in kdrl:GFP background. Note the increased number of BrdU and kdrl:GFP double positive cells within the DA in mCherry-negative she mutant embryos. Data combined from 2 independent experiments. *p<0.05, Student’s t-test.