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[Preprint]. 2023 Jul 5:2023.07.03.547455. [Version 2] doi: 10.1101/2023.07.03.547455

Figure 9. Inhibition of SHE in HUVECs results in enlarged tubulogenesis.

Figure 9.

(A-F) HUVEC cells were transfected with either control or SHE siRNA and analyzed in 3D collagen matrix assay at 48 (A-C) or 72 h (D-F) after transfection. The values (± standard deviation) are derived from analyzed fields (n=15) where total EC tube area was measured. Asterisks indicate significance at p<0.05 compared to control conditions.

(G,H) Western blots for expression of SHE and phosphorylation of PAK4, PAK2, ERK and CRKL in HUVEC cells transfected with a control or SHE siRNA. Note greatly reduced SHE expression and increased pPAK4 and pCRKL in cells transfected with siSHE RNA. (H) shows replicate experiments for pPAK4 and pCRKL.