Figure 3.

Putative biosynthetic gene cluster for hectoramide B. (a) Purple arrows indicate core biosynthetic genes. Additional arrows indicate additional ORFs that provide provisional boundaries to the hca BGC. Their proposed functions can be found in Table S9. (b) There are four core biosynthetic modules organized in a colinear fashion in the hca pathway. C: condensation domain, A: adenylation domain, TYR: adenylation domain for tyrosine incorporation, OMT: oxygen methyltransferase domain, NMT: nitrogen methyl transferase domain, MPOPA: adenylation domain for the proposed 3-(4-methoxyphenyl)-2-oxopropanoic acid incorporation, CAL: co-enzyme A ligase domain, KR: ketoreductase domain, VAL: adenylation domain for valine incorporation, AMT: amidotransferase. PCP: peptidyl-carrier protein. Phosphopantathiene arms are symbolized by wavy lines.