Figure 4.

Non-equivalent expression of costimulatory molecules by migratory cDC2s drives divergent T cell activation states. A-D) Naïve Ly5.2 4get.OT-II cells were transferred to Ly5.1 mice, injected with papain OVA or CpG OVA in the ear pinnae or front footpad, treated intraperitoneally with α-CD62L 6 hours post immunization, and dLNs were harvested and assessed by histo-cytometry or flow cytometry at 48 hours. A-C) Expression of IRF4 and BATF on activated CD44⁺ OT-II cells and correlation plots with Gata3 gMFI and frequency of 4get-GFP⁺ cells across different dLNs is shown. D) Representative histo-cytometry plots and quantification of pS6 and Gata3 expression in activated Ki67⁺IRF4⁺ OT-II T cells. E) Naïve Ly5.2 4get.OT-II cells were transferred to Ly5.1 mice and injected with a fixed concentration of papain and increasing concentrations of OVA in the ear pinnae or front footpad. Mice were treated intraperitoneally with α-CD62L 6 hours post immunization and dLNs were harvested and assessed by flow cytometry at 48 hours. CD44⁺ OT-II cell number, frequency of 4get-GFP⁺ cells, and Gata3, IRF4, and Tbet gMFI expression on CD44⁺ OT-II cells are shown. Data are representative of at least 3 independent experiments.