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Representative confocal images of isolated mouse hindlimb fibers immunostained with anti-Numb (green) (B and E) and anti-Septin 7 antibodies (red) (C-F); merge: merged images (A and D). Nuclei were labeled with DAPI (blue). A-C: myofiber from a control mouse (vehicle treated); D-F, myofiber from a Numb cKO mouse (tamoxifen treated). Scale bar, 10 μm.
