Table 3.
Fluorescence Correlation Spectroscopy, Time-Resolved Fluorescence and Anisotropy Parameters
| Atto488 Ext. Oligo Complex | Atto488 Int. Oligo Complex | |
|---|---|---|
| Overall Results | ||
| Molecular Brightness (kHz/molecule) | 8.1 | 13.5 |
| Species Average Lifetime (〈τ〉x) (ns) | 2.58 | 3.05 |
| Diffusion coefficient (μm2/s) | 100 | 120 |
| Quantum Yield | 50% | 60% |
| Time Resolved Fluorescence and Anisotropy Parameters | ||
| A1 | 57% | 68% |
| t1 (ns) | 4.0 | 4.0 |
| A2 | 43% | 32% |
| t2 (ns) | 0.76 | 0.92 |
| χ2 | 1.65 | 1.64 |
| Rotational Time (ps) | 280 | 350 |
| Fluorescence Correlation Spectroscopy | ||
| G0 | 1.00 | 1.00 |
| N | 0.49 | 0.50 |
| tdiff (ms) | 0.71 | 0.59 |
| ωo | 4.73 | 4.73 |
| A T 1 | 0.28 | 0.26 |
| tT1 (μs) | 2.4 | 0.9 |
| A T 2 | 0.13 | 0.082 |
| tT2 (ms) | 0.19 | 0.088 |
| χ2 | 0.96 | 0.86 |