Table 2. primerJinn output for eight drug resistance conferring gene regions of M. tuberculosis H37Rv (NC_000962.3).
Annealing temperatures (Tms) are in Celsius (Tms have been rounded for illustration purposes), and the product sizes in nucleotides.
| Forward Primer | Reverse Primer | Forward Tm | Reverse Tm | Product Size | Name | Forward Primer Tm NEB | Reverse Primer Tm NEB |
|---|---|---|---|---|---|---|---|
| GAGGAACACCACTAGTACCG | CTCGATGACTTTACGGCCAT | 65 | 64 | 675 | Target 1461045–1461291 | 64 | 64 |
| CATGGGATATGGAGCGATCG | GGGGTCGTAGGAGATCTTGA | 66 | 66 | 791 | Target 490900–491416 | 65 | 65 |
| CCGGTTGTCCATTCCGTTTA | CTGTACGTATTTGGGTTGCG | 64 | 66 | 454 | Target 1303831–1303911 | 65 | 64 |
| GGATGCGAGCTATATCTCCG | AATACGCCGAGAT GTGGAT G | 65 | 65 | 458 | Target 1674182–1674222 | 65 | 65 |
| CAACAGTTCATCCCGGTTCG | GACGGATTTGTCGCTCACTA | 65 | 67 | 759 | Target 2288681–2289242 | 66 | 64 |
| GCCACCATCGAATATCTGGT | GCTCCAGGAAGGGAATCATC | 66 | 65 | 778 | Target 761007–761277 | 64 | 65 |
| GCATACCGAACGTCACAGAT | ACGGTCACCTACAAAAACGG | 66 | 65 | 665 | Target 778990–779488 | 65 | 65 |
| GCTCTTAAGGCTGGCAATCT | CGGTCACACTTTCGGTAAGA | 65 | 66 | 577 | Target 2154831–2154873 | 65 | 64 |