Figure 2.

Analysis of cell-forming complexes following mesodermal differentiation using CLSM. A-L Representative images of 3 replicates show PBMC-derived iPSCs (A-F) or fibroblast-derived iPSCs (G-L) differentiated either according to an embryoid body-based (EB) protocol (A, B, C, G, H, I) or a monolayer-based (MB) protocol (D, E, F, J, K, L) and stained for ectodermal (A, D, G, J), mesodermal (B, E, H, K), and endodermal markers (C, F, I, L). Expression patterns of cell-forming complexes of PBMC-derived iPSCs demonstrate a higher degree of organization compared to complexes formed by fibroblast-derived iPSCs. Scale bar = 300 μm