Fig. 5. Effects of SCU on AKT and MAPK pathways in HepG2 cells. (a) Protein levels of p-AKT, p-p38, p-ERK1/2, and p-JNK analyzed by western blot in HepG2 cells after SCU (0, 20, 40, and 80 μmol/L) pretreatments for 1 h and ethanol (600 mmol/L) treatment for 24 h. (b) Quantification analyses of p-AKT, p-p38, p-ERK1/2, and p-JNK protein expression in (a). All values ( n=3) are demonstrated as mean±standard deviation (SD). ^*** P<0.001 versus control; ^### P<0.001 versus model. SCU: scutellarin; AKT: protein kinase B; MAPK: mitogen-activated protein kinase; p-AKT: phosphorylated AKT; ERK1/2: extracellular signal-regulated kinase 1/2; p-ERK1/2: phosphorylated ERK1/2; JNK: c-Jun N-terminal kinase; p-JNK: phosphorylated JNK; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.