Figure 5.

Guizhi Fuling capsule (GZFL) raises the expressions of synaptic proteins and decreases the phosphorylation of tau protein in the hippocampus (HPC) of presenilin1/2 conditional double knockout (PS cDKO) mice. The brain tissues of mice in each group were lysed, quantified and then analyzed by Western blot to detect the expressions of synaptic proteins (NR2A, NR2B, PSD95, MAP2 and SYP) and phosphorylated tau protein (ser396/404). (A) Representative Western blot for synaptic proteins in HPC. (B–F) Quantification of Western blot for synaptic proteins in HPC. (G) Representative Western blot for phosphorylated tau proteins in HPC. (H, I) Quantification of Western blot for phosphorylated tau proteins in HPC. The protein expressions of synaptic proteins and phosphorylated tau proteins were standardized based on the respective expressions of β-actin or total tau. Uncropped immunoblots are shown in Supplementary Figure 2 . These values were showed as relative changes to the respective wild-type (WT) mice, which was set to 1. Data are the mean ± SEM (n = 6), *P < 0.05; **P < 0.01.