Figure 4.

Expression of Pattern Recognition Receptors (PRRs) in the originally established porcine bronchial epithelial (PBE) cell line. PBE cells were seeded at an initial concentration of 1.0 x 10⁴ cells/cm² and evaluated at day 6. The expressions of PRRs from the Toll-like receptor (TLR) and the nucleotide-binding oligomerization domain-containing protein (NOD) families were evaluated by qPCR. Results are expressed as copy numbers of the PRRS genes per 25 ng of cDNA. Photographs show the PBE cells stained with fluorescent antibodies directed to TLR3, TLR4, or TLR7. Cell nuclei were stained with DAPI. Results represent data from three independent experiments.