Figure 7.

Expression of inflammatory cytokines and chemokines in the originally established porcine bronchial epithelial (PBE) cell line in response to the activation of the Toll-like receptor 3 (TLR3) signaling pathway. PBE cells were seeded at an initial concentration of 1.0 x 10⁴ cells/cm². At day 6, PBE cells were stimulated with the TLR3 synthetic agonist poly(I:C) (100 ng/ml) and the expressions of the inflammatory factors TNF-α, IL-6, IL-8, and MCP-1 were evaluated by qPCR at the indicated time points. Results represent data from three independent experiments. Asterisks indicate significant differences between the control and the poly(I:C)-treated PBE cells. * (P < 0.05), ** (P < 0.01).