Figure 6.

Quantitative assessment of dendritic complexity of Thy1-EGFP expressing mouse subicular by Sholl analysis. Dendrites of subicular pyramidal neurons were separated into apical and basal arbors with regard to their origin. (A) Sholl analysis of apical dendritic arborization revealed significantly higher level of complexity following neonatal sevoflurane treatment in both males and females, as evidenced by the measurement of sum intersections, total number of crossings at all Sholl radii (Left panel), and max intersections, highest number of Sholl crossings ay any radii (Right panel). The observed increase in complexity impacted sevoflurane-treated males more significantly than female counterparts. (B) On the contrary, increased complexity in basal dendritic arborization was detected only in sevoflurane-treated female mice, with significant increases in both sum intersections (Left panel) and max intersections (Right panel). No changes were observed as a result of treatment in males in either parameter. *p < 0.05; **p < 0.01; ***p < 0.001.

EGFP, enhanced green fluorescent protein.