Table 4.
Miscellaneous parameters of Sholl analysis performed separately on apical and dendritic arborization of subicular pyramidal neurons in juvenile mice. Neonatal sevoflurane induced significant increase in enclosing radius in males, indicating a wider apical dendritic arbor. Furthermore, ramification index in both males and females compared with controls, signifying higher ratio between maximum intersections and primary branches. No difference was noted in other parameters.
| Males (M) | Females (F) | C versus S (males) |
C versus S (females) |
||
|---|---|---|---|---|---|
| Apical dendrite Sholl – mouse | Mean ± SD | Mean ± SD | Significance | ||
| Max intersections radius | Control (C) | 78.06 ± 18.56 | 80.19 ± 25.76 | p = 0.96 | p = 0.29 |
| Sevoflurane (S) | 79.15 ± 19.97 | 86.57 ± 23.01 | |||
| Enclosing radius | Control | 27.23 ± 4.07 | 28.17 ± 3.92 | *p = 0.04 | p = 0.72 |
| Sevoflurane (S) | 29.06 ± 3.47 | 28.74 ± 3.92 | |||
| Ramification index | Control | 8.70 ± 3.20 | 7.63 ± 3.41 | **p < 0.01 | **p < 0.01 |
| Sevoflurane (S) | 10.98 ± 3.40 | 9.86 ± 3.71 | |||
| Sholl decay | Control | 0.93 ± 0.04 | 0.93 ± 0.04 | p = 0.06 | p = 0.19 |
| Sevoflurane (S) | 0.91 ± 0.04 | 0.92 ± 0.04 | |||
| Basal dendrite Sholl – mouse | Mean ± SD | Mean ± SD | Significance | ||
| Max intersections radius | Control | 47.99 ± 16.19 | 41.86 ± 15.53 | p = 0.88 | p = 0.39 |
| Sevoflurane (S) | 46.50 ± 16.05 | 45.90 ± 16.03 | |||
| Enclosing radius | Control | 23.86 ± 3.11 | 22.61 ± 3.13 | p = 0.91 | p = 0.65 |
| Sevoflurane (S) | 23.62 ± 2.87 | 23.12 ± 2.94 | |||
| Ramification index | Control | 4.46 ± 2.41 | 3.82 ± 1.28 | p = 0.94 | p = 0.10 |
| Sevoflurane (S) | 4.34 ± 1.37 | 4.52 ± 1.83 | |||
| Sholl decay | Control | 0.97 ± 0.02 | 0.97 ± 0.01 | p = 0.78 | p = 0.95 |
| Sevoflurane (S) | 0.97 ± 0.01 | 0.97 ± 0.01 | |||
SD: standard deviation.