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. 2023 Jun 2;42(14):e113110. doi: 10.15252/embj.2022113110

Figure EV2. Cryo EM data and map validation for p97.

Figure EV2

  • A–C
    Example raw images from the particle dataset. Scale bar, 500 Å.
  • D
    Example 2D class averages, showing some different particle orientations, with some weak substrate densities adjacent to the stronger p97 features. The box size is 352 × 352 Å.
  • E
    Gold‐standard Fourier shell correlation (GSFSC) calculated during refinement without and with mask. The resolutions were determined at FSC = 0.143 (dotted line).
  • F
    Plot of particle orientations.
  • G
    Local resolution was calculated from the half‐map and colored according to the scale on the side. p97 subunits A–F labeled. For this map 866,937 particles were used.