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. 2023 May 30;42(14):e113168. doi: 10.15252/embj.2022113168

Figure EV3. Cooling represses RBM3 poison exon inclusion. Related to Fig 3 .

Figure EV3

  1. PSI values of RBM3 Exon 4 relative to Exon 3 and 6, and Exon 5 relative to Exon 4 and 6 in i‐neurons at 37 or 32°C (72 h). N = 4 biological replicates.
  2. RT‐PCR of RBM3 minigene expressed in HeLa cells at 37 and 32°C (48 h) in the presence or absence of cycloheximide (CHX) at 200 μg/ml concentration. PSI values of RBM3 PE are calculated based on the intensity of PE‐included (red arrows) and PE‐skipped (green arrow) isoforms. N = 1 biological replicate.

Data information: Mean ± SEM; n.s. (not significant); FDR calculated by rMATS program in (A).

Source data are available online for this figure.