Figure 4. Xkr8 deficiency limits microglial uptake of presynaptic material in developing S1 cortex.

• A–F
  The immunolabeling of internalized pan‐axonal material (A, B), pre‐synaptic vGluT1 particles (C, D) and post‐synaptic PSD95 particles (E, F) within Iba1⁺ microglia in P8 cortex of Xkr8 WT and Xkr8 cKO mice was quantified per individual 3D reconstructed microglial cell. The total volume of internalized particles was normalized to microglial cell volume and compared by nested design and mixed model ANOVA, each dot represents an individual mouse (n = 3 per genotype group). Data presented as mean ± SEM; **P < 0.01, ****P < 0.0001; scale bar 10 μm, grid cell 10 μm.

Source data are available online for this figure.