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. 2023 May 25;42(14):e112817. doi: 10.15252/embj.2022112817

Figure EV2. B. abortus induces HIF‐1α stabilisation in a hypoxia‐independent manner.

Figure EV2

  • A–C
    Representative confocal micrographs of HeLa cells infected or not with B. abortus 544 GFP (red) for 24 h (A), 48 h (B) and 72 h (C), treated with 150 μM of the EF5 compound for 3 h before analysis, then fixed and immunostained for EF5 (Anti‐EF5 Cy5 conjugate—magenta) and HIF‐1α (Alexa 568—green). DNA was stained with Hoechst 33258 (blue). Scale bars: 20 μm. Figure panels EV2A–C, reuse the same experiment as described in Fig 3A.
  • D
    Representative confocal micrographs of HeLa cells treated with 150 μM of the EF5 compound, exposed to normoxia (21% O2), hypoxia (1% O2) or an intermediate hypoxia (between 21% and 1% O2) for 3 h, then fixed and immunostained for EF5 (anti‐EF5 Cy5 conjugate—green). DNA was stained with Hoechst 33258 (blue). Scale bars: 20 μm.

Source data are available online for this figure.