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. Author manuscript; available in PMC: 2023 Jul 17.
Published in final edited form as: Biomater Adv. 2021 Nov 29;134:112576. doi: 10.1016/j.msec.2021.112576

Table 1.

List of various functionalized polymeric biomaterials, photoinitiators, photocrosslinking parameters for 3D bioprinting of biological constructs.

Biomaterials Photoinitiator Photocuring parameters Printed construct Cell types used Ref.

Methacrylated collagen (MeCol), Alginate coated Carbon nanotubes (CNT) Irgacure 2959 Light source: UV
Wavelength: 365 nm
Irradiation time: 45 s
Intensity: 6.1 mW/cm2
3D bioprinted cardiac patch Human coronary artery endothelial cells (HCAECs) [18]
PEG norbornene macromers Lithium phenyl-2,4,6-trimethyl-benzoyl phosphinate (LAP) Light source: UV
Wavelength: 365 nm Irradiation time: 3 min
Intensity: 60 mW/cm2
Ear shaped construct Human mesenchymal stem cells (hMSCs) [19]
Methacrylated gelatin (GelMA), poly(γ-glutamic acid) Irgacure 959 Light source: UV
Irradiation time: 30 s Intensity: 8 mW/cm2
Single-layer cylindrical construct and double-layer tubular construct Human umbilical vascular endothelial cells (HUVECs), smooth muscle cells (HSMCs) [20]
Silk fibroin, Gelatin Ruthenium (Ru), Sodium per sulfate (SPS) Light source: Visible light
Wavelength: 400–450 nm
Irradiation time: 3 min
Intensity: 30 mW/cm2
Grid pattern (12 × 12 mm), 7 layered constructs Human articular chondrocytes [21]
GelMA, tyramine Ruthenium/sodium per sulfate Light source: Visible light
Wavelength: 450 nm
Grid pattern Articular chondroprogenitor cells [22]
κ-carrageenan Eosin Y Light source: Visible light
Intensity: 100 mW/cm2
Irradiation time: 180 s
Rectangular mold (15 × 5 × 1 mm) HeLa and fibroblasts [23]
Norbornene-functionalized neutral soluble collagen LAP Light source: UV
Wavelength:365 nm
Intensity: 5 mW/ cm2
Irradiation time: 30 s
The pyramid side length of 9.2 mm and a height of 5 mm, monolayer tube with a diameter of 8 mm and a height of 10 mm. Human umbilical vein endothelial cells [24]
Gelatin methacrylate Irgacure 2959 Light source: visible light laser
Wavelength: 405 nm
Intensity: 1–5 mW/mm2
Irradiation time: 10 s
Microtube pattern NIH 3T3 fibroblast cells [25]
Gelatin methacryloyl (GelMA) Eosin Y, triethanol amine, N-vinyl caprolactam Light source: Visible light
Wavelength: 400–750 nm
Intensity: 48.6 mW cm−2
Irradiation time: 60 s
Irradiation distance: 10 cm
Honeycomb pattern and miniaturized multi-layered human ear and cross-section of the brain 3T3 fibroblasts, U118 astrocytes [26]
Methacrylated auricular cartilage-derived decellularized extracellular matrix (cdECMMA), gelatin, glycerol, hyaluronic acid Irgacure 2959 Light source: UV light
Light intensity: 200 mW/cm2
Irradiation time: 2 min
Lattice and ear-shaped cell-laden constructs Rabbit auricular chondrocytes [27]
Oligo (poly(ethylene glycol) fumarate) (OPF), gelatin, polyethylene glycol diacrylate (PEGDA) Irgacure 2959 Light source: UV light
Irradiation time: 2 min
Grid pattern with 10 mm × 10 mm width and length with 0.25 mm single layer thickness and 25% infill density MC3T3 pre-osteoblast cells and PC12 cells [28]
Methacryloyl gelatin, new Coccine LAP Light source: visible light
Wavelength: 405 nm
Light source working parameters: 200 mW & 3.3 V
Grid structures with different porosities, thin-walled text structure (“EFL”), large inclined structure, thin-plate liver unit structure, and truss liver unit structures Mouse bone marrow mesenchymal stem cells (mBMSCs) [29]
Methacrylated gelatin, hyaluronic acid glycidyl methacrylate, polyethylene glycol diacrylate LAP Light source: UV light
Wavelength: 365 nm
Intensity: 11 mW/cm2
Irradiation time: 45 s
Slab, line, grid, dispersion, and random patterns Neonatal mouse ventricular cardiomyocytes (NMVCMs) [30]
Recombinant methacrylated collagen type I (RCPhC1-MA), Recombinant norbornene collagen type I (RCPhC1-NB), Recombinant thiolated collagen type I (RCPhC1-SH) Tetrapotassium 4,4′-(1,2-ethenediyl)bis [2-(3-sulfophenyl)diazenesulfonate] (DAS) Light source: visible light laser
Wavelength: 720 nm
Laser power: 10–100 mW
Cube-shaped patterns (200 × 200 × 200 μm) and different logos (TU Wien, B-PHOT, and PBM) TERT-immortalized human adipose tissue – derived stem cell line (ASC/TERT1) [31]