Fig. 4.

RyR2 post-translational modifications are aging associated in human cerebellum. a Representative western blot of immunoprecipitated RyR2 macromolecular complex in the postmortem human ET and control cerebellum. Quantification of blots for b p-RyR2/pS2808, c DNP/RyR2, d Cys-NO/RyR2 and e Calstabin2/RyR2 (n = 6 young controls, n = 6 old controls, n = 12 ET). The RyR2 macromolecular complex is post-translationally modified in old controls (> 60 years) and ET patients (youngest ET 67 years) but not in young controls (< 59 years) [One-way ANOVA with Tukey’s multiple comparisons ****p < 0.0001]. f Linear regression (r = 0.7747) and Spearman correlation (p < 0.0001) of calstabin2 depletion from RyR2 (Calstabin2/RyR2) with age, indicating a significant correlation between RyR2 remodeling and age, which occurs after age 60 years