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. 2023 Jul 17;54:60. doi: 10.1186/s13567-023-01191-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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A schematic diagram of the construction of the BAC-CHv-ΔUL49 virus on the BAC platform. (I) The genome structure of DPV CHv. (II) The first Red recombination, the UL49 gene was replaced by the Kan resistance gene. (III) The product after the first Red recombination. (IV) The second Red recombination, the I- SecI cleavage site was recognized by the recombinase, and the Kan fragment was removed. (V) The genome structure of BAC-CHv-ΔUL49.