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. 2023 Jul 17;54:60. doi: 10.1186/s13567-023-01191-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Multi-step growth curves. DEF cells in a 24-well plate were infected with BAC-CHv, BAC-CHv-UL49R, and BAC-CHv-ΔUL49 at an MOI of 0.01. The supernatant and cell samples were collected at the specified time points, respectively, and viral titers were measured. Data were calculated as mean ± standard deviation (SD, P > 0.05). A Virus titer of supernatant. B Virus titer of cells. C Statistical analysis of virus titer difference in the supernatant at each time point. D Statistical analysis of differences in cell virus titers at each time point. The asterisk indicates a significant difference compared to the BAC-CHv and BAC-CHv-UL49R viruses (^∗∗∗∗P < 0.0001; ^∗∗∗P < 0.001; ^∗P < 0.05).