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. 2023 Jul 17;42:170. doi: 10.1186/s13046-023-02754-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characteristics of the CRC-derived PDOs. Patient-Derived-Organoids were obtained from four right colon adenocarcinoma specimens as described in methods. a Right panel: Clinico-pathological features of the obtained specimens. Left panel: representative micrographs of the four PDO cultures obtained from the specimens indicated in 3a, left. Size bar: 200 µm. b Validation of the PDO cultures. Upper panel: histograms showing the percentage of cells positive for EpCAM, Ki67, CD44 and CK20 in the CRC tissue immediately after the mechanical disaggregation (passage 0, p0). Lower panel: histograms showing the percentage of cells positive for the expression of the above antigens in PDO cultures disaggregated after three sequential passages (passage 3, p3). c High correlation between the number of positive cells within the p0) and the p3) specimens was shown (r = 0.8213, p < 0.01) suggesting a similar composition in cell subpopulations between the p0 and the p3 PDOs