Fig. 2.

Mandalorion performance is least affected by the absence of annotation. Recall and precision of Mandalorion (M), StringTie (S), Bambu (B), and IsoQuant (I) are shown for simulated and real PacBio data. Recall and precision are shown for tools run with (A, “w/A”) or without (B, “w/o A”) annotation. Recall is also shown with more stringent distance thresholds applied