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. 2023 Jul 17;21:475. doi: 10.1186/s12967-023-04303-3

Fig. 1.

Fig. 1

Identification of primary CAFs and NFs. A qRT-PCR results shows that the mRNA expression levels of FAP, FSP1, α-SMA, and CD90 in CAFs are significantly higher than those in NFs and T24 cells. B, C WB verified that the protein expression levels of α-SMA and Vimentin in CAFs are significantly higher than those in NFs and T24 cells. DG Immunofluorescence staining shows that CAFs and NFs have an elongated spindle morphology, and CAFs express more Vimentin and α-SMA than NFs