Fig. 6. Notch signaling is activated in cells grown on stiffness ECM or treated with Exo^stiff.

a, Huh7 cells grown on 0.5 or 10 kPa matrix were lysed and the cell lysates were subject to immunoblotting for Notch signaling proteins Sox9, c-Myc, Hes1, and Jagged1. GAPDH was used as a loading control. Molecular weights (in kDa) are shown to the right. b, Huh7 cells grown on the soft matrix were treated with Exo^soft or Exo^stiff. Cell lysates were subjected to immunoblotting with antibodies against Notch pathway proteins. GAPDH was used as a loading control. c, Immunohistochemistry of tumors from mice treated with Exo^soft or Exo^stiff. Tumor tissues were harvested at Day 27 and stained with H&E, Sox9, or Hes1. Scale Bar: 100μm. The percentages of Sox9 and Hes1-positive cells in mouse xenografts were shown at the right. Values are presented as Mean ± S.D., n=5. Source numerical data and unprocessed blots are available in source data. n represents the number of animals.