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. Author manuscript; available in PMC: 2023 Jul 17.
Published in final edited form as: Nat Cell Biol. 2023 Feb 16;25(3):415–424. doi: 10.1038/s41556-023-01092-1

Fig. 7. Jagged1 is enriched in Exostiff.

Fig. 7.

a, b, Exosomes were purified from the conditioned media of Huh7 (a) or Hepa1-6 cells (b) on soft or stiff matrix. The same amounts of exosomes were loaded for immunoblotting of Jagged1, Syntenin-1, HRS, CD63, Tsg101. The quantification is shown at the right. (n=3 independent experiments) c, Jagged1 in Huh7 cells grown on soft and stiff matrix were co-immunostained with antibodies against CD63 or Flag-tagged HRS for fluorescence imaging. Scale Bar: 10μm. Zoom-in view Scale bar: 1μm.d, Pearson’s correlation coefficient of Jagged1 colocalized with CD63 (n=15) or HRS (n=17). n represents the number of cells. e, HA-tagged Jagged1 was co-transfected with Flag-tagged HRS or vector into cells. The cell lysates were subjected to immunoprecipitation with anti-Flag M2 beads. Isotype IgG and anti-Myc epitope antibodies were used as negative controls. For all figures above, values are presented as Mean ± S.D. Source numerical data and unprocessed blots are available in source data.