Figure 5. Microbiota and TLR signaling are dispensable for CD103^int DCs.

A. Representative plots (gated on Lin⁻CD64⁻CD11c⁺MHCII⁺ cells) and frequencies (mean ± SD) of DC subsets in total live cells in the lungs of 7–day old C57BL/6 mice housed in conventional or GF conditions. B. Representative histograms of CCR7 expression on CD103^int DCs and graphs of gMFI of CCR7 on DC subsets from the lungs of 7–day old C57BL/6 mice housed in conventional or GF conditions. C. Representative plots (gated on Lin⁻CD64⁻CD11c⁺MHCII⁺ cells) and frequencies (mean ± SD) of DC subsets in total live cells in the lungs of 7–day old C57BL/6 and Myd88^−/− mice. D. Representative histograms of CCR7 expression on CD103^int DCs and graphs of gMFI of CCR7 on DC subsets from the lungs of 7–day old C57BL/6 and Myd88^−/− mice. E. Representative plots (gated on Lin⁻CD64⁻CD11c⁺MHCII⁺ cells) and frequencies (mean ± SD) of DC subsets in total live cells in the lungs of 7–day old C57BL/6 and Trif^−/− mice. F. Representative histograms of CCR7 expression on CD103^int DCs and graphs of gMFI of CCR7 on DC subsets from the lungs of 7–day old C57BL/6 and Trif^−/− mice. These experiments were performed 2 times for each comparison with 3 mice (A–B), 3 mice (C–D) and 5 mice (E–F) per group. Significance was determined using 2-way ANOVA with Sidak multiple comparision test. *p<0.05, ***p<0.001, ****p<0.0001. G. Representative plots (gated on Lin⁻CD64⁻CD11c⁺MHCII⁺ cells) and frequencies (mean ± SD) of DC subsets in the lungs of 7–day old C57BL/6, ST2^−/−, plt/plt, Ccr7^−/−, Ccr2^−/− mice and CD11c-Ctnnb1^−/− mice. H. Representative histograms of CCR7 expression and frequency (mean ± SD) of CCR7+ cells in DC subsets. Dashed lines divide marker⁺ from marker⁻ cells. These experiments were performed at least 2 times with 3 C57BL/7, 4 ST2^−/−, 3 plt/plt, 3 Ccr7^−/−, 3 Ccr2^−/− mice and 6 CD11c-Ctnnb1^−/− mice.