C-di-GMP effects on aerotactic band formation in A. brasilense. a, Aerotactic band formation after introduction of an oxygen gradient in the wild-type strain Sp7 containing an empty vector pIND4. Two parameters, distance from the band to the meniscus (d) and band width (w) are used to characterize oxygen sensitivity. b, The red light-activated DGC, BphS, expressed from pRed-DGC increases band thickness. c, The blue light-activated PDE, EB1, expressed from pBlue-PDE changes band position in regard to the meniscus in the tlp1 mutant.