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. 2023 Feb 22;19(8):2372–2385. doi: 10.1080/15548627.2023.2178159

Figure 2.

Figure 2.

Screening small compound libraries. Results of 41,161 compounds (originating from Prestwick, Selleck, MicroSourse, LOPAC, and ChemDiv libraries) screened in the PCA high-throughput system. Blue dots represent ATG12-L1 WT + ATG3-L2 lysates + DMSO solvent (no compound, used as negative control, normalized to 0). Red dots represent ATG12-L1K54D + ATG3-L2 lysates + DMSO solvent (used as positive control for maximal inhibition, normalized to −100). Black dots each represent ATG12-L1 WT + ATG3-L2 lysates + library compounds (10 µM). Dashed red line represents the threshold (20% inhibition). Total screen hit rate is 1.9%, and Z’ score is 0.76. X-axis corresponds to the well number, corresponding to all three types of samples (controls and compounds).