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. 2023 Feb 22;19(8):2372–2385. doi: 10.1080/15548627.2023.2178159

Figure 6.

Figure 6.

Compound 189 reduces IL1B secretion in LPS-treated macrophage-like cells. (A) RAW 264.7 cells were treated with LPS (1 μg/ml) combined with DMSO, compound 189 (10 μM), or dexamethasone (2 μg/ml) for 24 h, and levels of IL1B in the medium was measured by ELISA. Data represent mean ± SD of 6 biological repetitions, and was normalized to LPS-treated cells. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test, **P < 0.01; ****P < 0.0001. (B) THP-1 cells were treated with 0.5 μM PMA for 3 h followed by 0.5 μg/ml LPS combined with DMSO, compound 189 (10 μM), or dexamethasone (2 μg/ml) for 4 h and IL1B levels were assayed by ELISA as in A. Data represent mean ± SD of 3 biological repetitions, and was normalized to LPS-treated cells. Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test, ***P < 0.001; ****P < 0.0001.