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. 2023 Jul 17;131(7):077006. doi: 10.1289/EHP12259

Figure 5.

Figure 5A is a stained tissue with three columns, namely, concentration of water, exposure of water, and exposure of nicotinamide mononucleotide and three rows, namely, S100a9, S100a8, and ll1b. Figure 5B is a stained tissue with three columns, namely, concentration of water, exposure of water, and exposure of nicotinamide mononucleotide and four rows, namely, Mmp8, Col3a1, Col1a1, Fn1. Figures 5C and 5D are Rose diagrams titled particulate matter differential expressed genes (concentration of water versus exposure of water) and nicotinamide mononucleotide differential expressed genes (exposure of water versus exposure of nicotinamide mononucleotide) displays twelve cell types, namely, alveolar macrophages, neutrophils, b cells, t cells, monocytes, dividing dendritic cells, N K cells, fibroblasts, club cells, conventional dendritic cells, A T 2, and A T 1. A scale ranges from 0 to 400 in increments of 100, respectively.

Expression of pro-inflammatory and pro-fibrotic marker genes and illustration of DEGs (A) Expression levels of pro-inflammatory genes including Il1b, S100a8, and S100a9 on UMAP plots split by Con-H2O, Exp-H2O, and Exp-NMN groups. (B) Expression levels of profibrotic genes including Fn1, Col1a1, Col3a1, and Mmp8 on UMAP plots split by Con-H2O, Exp-H2O, and Exp-NMN groups. The numbers of (C) “PM Exposure DEGs” (Exp-H2O vs. Con-H2O) and (D) “NMN Treatment DEGs” (Exp-NMN vs. Exp-H2O) identified in each cell cluster are displayed in rose diagrams. The corresponding data of (A) and (B) are listed in Excel Table S9. The detailed information of the genes summarized in (C) and (D) are provided in Excel Tables S4 and S5. Note: AM, alveolar macrophages; AT1, type 1 alveolar epithelial cells; AT2, type 2 alveolar epithelial cells; Con, air-filtered control group; DC, dendritic cells; DEGs, differentially expressed genes; dividing DC, dividing dendritic cells; Exp, PM exposure group; H2O, water; Monocytes, monocyte-derived cells; NK, NK cells; NMN, nicotinamide mononucleotide; PM, particulate matter; UMAP, Uniform Manifold Approximation and Projection.