KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| β-Actin | Cell Signaling Technologies | Cat#4970, RRID:AB_2223172 |
| HA tag | Cell Signaling Technologies | Cat#3724, RRID:AB_1549585 |
| DsDNA | Abcam | Cat#ab27156, RRID:AB_470907 |
| Histone H3 (tri-methyl K9) | Abcam | Cat#ab8898, RRID:AB_306848 |
| β1-integrin | MilliporeSigma | Cat#AB1952, RRID:AB_91150 |
| α-tubulin | MilliporeSigma | Cat#T9026, RRID:AB_477593 |
| CD63 | System Biosciences International | Cat#EXOAB-CD63A-1, RRID:AB_2561274 |
| VAMP3 | ProteinTech | Cat#107021-AP, RRID:AB_2212628 |
| ARF6 | ProteinTech | Cat#20225-1-AP, RRID:AB_10646481 |
| Emerin | ProteinTech | Cat#10351-1-AP, RRID:AB_2100056 |
| APEX1 | ProteinTech | Cat#10203-1-AP, RRID:AB_2057927 |
| Annexin-A1 | ProteinTech | Cat#21990-1-AP, RRID:AB_11182596 |
| Flotillin | ProteinTech | Cat#15571-1-AP, RRID:AB_2106746 |
| Alix | ProteinTech | Cat#12422-1-AP, RRID:AB_2162467 |
| CD81 | ProteinTech | Cat#66866-1-Ig, RRID:AB_2882203 |
| TSG-101 | ProteinTech | Cat#28283-1-AP, RRID:AB_2881104 |
| Histone-H3 | ProteinTech | Cat#17168-1-AP, RRID:AB_2716755 |
| CGAS | ProteinTech | Cat#26416-1-AP, RRID:AB_2880507 |
| CD63 | Developmental Studies Hybridoma Bank | Cat#h5c6, RRID:AB_528158 |
| SRY | ProteinTech | Cat#67349-1-lg, RRID:AB_2882607 |
| GFP | ThermoFisher | Cat#33-2600, RRID:AB_2533111 |
| Donkey anti-mouse Alexa Fluor Plus 488 | ThermoFisher | Cat#A32766, RRID:AB_2762823 |
| Donkey anti-rabbit Alexa Fluor Plus 555 | ThermoFisher | Cat#A32794, RRID:AB_2762834 |
| Donkey anti-mouse Alexa Fluor Plus 680 | ThermoFisher | Cat#A32788, RRID:AB_2762831 |
| Donkey anti-rabbit Alexa Fluor Plus 800 | ThermoFisher | Cat# A32808, RRID:AB_2762837 |
| Bacterial and virus strains | ||
| NEB Stable | New England Biolabs | Cat#C3040I |
| Biological samples | ||
| Control human genomic DNA | ThermoFisher | Cat#360486 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor 555 Plus phalloidin | ThermoFisher | Cat#A30106 |
| Alexa Fluor 647 Plus phalloidin | ThermoFisher | Cat#A30107 |
| To-pro-3 iodide | ThermoFisher | Cat#T3605 |
| DRAQ-5 | ThermoFisher | Cat#62254 |
| Protease Inhibitor Cocktail | MilliporeSigma | Cat#P8340-5ML |
| Paraformaldehyde | Electron Microscopy Sciences | Cat#15713-5 |
| Poly-L-lysine | MilliporeSigma | Cat#P4832-50ML |
| Poly-HEMA | MilliporeSigma | Cat#P3932-10G; CAS#25249-16-5 |
| Matrigel | Corning | Cat#354230 |
| Horse Serum | ThermoFisher | Cat#16050122 |
| EGF | Peprotech | Cat#AF10015 |
| Hydrocortisone | MilliporeSigma | Cat#H0888-1G |
| Cholera Toxin | MilliporeSigma | Cat#C8052-2MG |
| Insulin | MilliporeSigma | Cat#I1822-100MG |
| B27 | ThermoFisher | Cat#17504044 |
| Glutaraldehyde (electron microscopy grade) | Electron Microscopy Sciences | Cat#16120 |
| GTP-γ-S | MilliporeSigma | Cat#G8634-1MG; CAS# 94825-44-2 |
| GDP-β-S | MilliporeSigma | Cat# G7637-5MG; CAS# 97952-36-8 |
| MG132 | MilliporeSigma | Cat#M8699-1MG; CAS# 1211877-36-9 |
| Chloroquine phosphate | Cayman Chemical | Cat#14194; CAS# 50-63-5 |
| U0126 | Cayman Chemical | Cat#70970; CAS# 109511-58-2 |
| Sulforhodamine B | MilliporeSigma | Cat#230162-5G; CAS# 3520-42-1 |
| Trichloroacetic Acid | MilliporeSigma | Cat#T6399-250G; CAS# 76-03-9 |
| Triton-X100 | Fisher Scientific | Cat#BP151-100 |
| Critical commercial assays | ||
| DNeasy Blood and Tissue Kit | Qiagen | Cat#69504 |
| BCA Protein Assay Kit | ThermoFisher | Cat#23227 |
| Ready Probes Cell Viability Imaging Kit | ThermoFisher | Cat#R37609 |
| Quant-iT High Sensitivity dsDNA Assay Kit | ThermoFisher | Cat#2129422 |
| SuperScript IV First-Strand Synthesis System with ezDNAse | ThermoFisher | Cat#18091150 |
| Monarch Total RNA Miniprep Kit | New England Biolabs | Cat#T2010S |
| Click-iT EdU Alexa Fluor 488 Cell Proliferation Kit | ThermoFisher | Cat#C10086 |
| Deposited data | ||
| The Cancer Genome Atlas | http://cancergenome.nih.gov/ | RRID:SCR_003193 |
| Experimental models: Cell lines | ||
| LOX | Fodstad et al. 1988 | N/A |
| H460 | ATCC | Cat#HTB-177, RRID:CVCL_0459 |
| MDA-MB-231 | ATCC | Cat#CRL-12532, RRID:CVCL_0062 |
| MDA-MB-468 | ATCC | Cat#HTB-132, RRID:CVCL_0419 |
| T-47D | ATCC | Cat#HTB-133, RRID:CVCL_0553 |
| PC-3 | ATCC | Cat#CRL-1435, RRID:CVCL_0035 |
| A375-P | ATCC | Cat#CRL-3224, RRID:CVCL_6233 |
| A375-MA2 | ATCC | Cat#CRL-3223, RRID:CVCL_X495 |
| 293FT | ThermoFisher | Cat#R70007 |
| DU145 | ATCC | Cat#HTB-81, RRID:CVCL_0105 |
| LNCaP | ATCC | Cat#CRL-1740, RRID:CVCL_1379 |
| MCF-10A | Joan Brugge | N/A |
| SW480 | ATCC | Cat#CCL-228, RRID:CVCL_0546 |
| Oligonucleotides | ||
| For complete list of oligonucleotides please see Table S1 | N/A | |
| Recombinant DNA | ||
| pEGFP-C1 | Clontech | Cat#6084-1 |
| LentiCRISPR v2 | Sanjana et al. 2014 | Addgene plasmid #52961; RRID:Addgene_52961 |
| psPAX2 | Didier Trono | Addgene plasmid #12260; RRID:Addgene_12260 |
| pMD2.G | Didier Trono | Addgene plasmid #12259; RRID:Addgene_12259 |
| pLKO.1-TRC | Moffat et al., 2006 | Addgene plasmid #10878; RRID:Addgene_10878 |
| pGEX-3X GST-wt-ARF6 | Schweitzer and D’Souza-Schorey 2005 | N/A |
| Software and algorithms | ||
| FIJI | www.imagej.net | Version 2.0.0-rc-69/1.52p |
| Graphpad Prism | www.graphpad.com | Version 9.2.0 |
| Microsoft Excel | https://www.microsoft.com/en-us/microsoft-365/excel | Version 16.16.27 |
| Histogram | Apogee Flow Systems; www.apogeeflow.com | N/A |
| UALCAN | Chandrashekar et al. 2017 | RRID:SCR_015827 |
| UCSC Xena | Goldman et al., 2020 | RRID:SCR_018938 |
| Other | ||
| Glutathione high capacity magnetic beads | MilliporeSigma | Cat#G0924-1ML |
| Exosome depleted FBS | ThermoFisher | Cat#A27208-01 |
| EquaFetal Serum | Atlas Biologicals | Cat#EF-0500-A |
| Hyclone FBS | Cytiva Life Sciences | Cat#SH30070.03 |
| Benzonase | MilliporeSigma | Cat#71206 |
| Immobilon PVDF-FL | MilliporeSigma | Cat#IPFL85R |
| Prolong diamond antifade mounting media | ThermoFisher | Cat#P36970 |
| Lipofectamine2000 Transfection Reagent | ThermoFisher | Cat#11668-027 |
| Micro flow cytometry calibration beads (Apogee Mix) | Apogee Flow Systems | Cat#1493 |
| Optiprep Density Gradient Medium | MilliporeSigma | Cat#D1555-250ML |
| ddPCR Supermix for probes (no dUTP) | BioRad | Cat#1863024 |
| TaqMan Fast Advance Master Mix | ThermoFisher | Cat#4444554 |