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. 2023 Jul 5;17(7):e0011470. doi: 10.1371/journal.pntd.0011470

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© 2023 Rattaprasert et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) LFD assay for RPA-amplified products at 39°C for 20 min from different Plasmodium species. Strips 1 and 2 contain the DNA-free template and 1 ng of P. cynomolgi plasmid DNA, respectively. Strips 3–7 consist of 5 ng genomic DNA of P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi, respectively. Strips 8 and 9 contain 1 ng of P. inui asexual and sexual-type recombinant plasmid DNA. (B) AGE results for the identical RPA amplicons amplified under the same conditions after purification. Lane M is a 100 bp molecular ladder (Thermo Fisher Scientific Inc., USA).