FIGURE 4.
T. brucei tRNATyr has an unusually short half-life, which is not shared by other tRNAs in the system. (A) Triplicate growth curve of WT T. brucei cells subjected to Actinomycin D treatment where total RNA was collected at the time points indicated. (B,C) Linear regression calculations utilizing the amounts of mature and alternate conformer tRNATyr. (D) Linear regression calculations utilizing the amounts of other tRNAs. Data were fit to a one-phase decay. All data plotted were the result of three biological replicas.