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. 2023 Aug;29(8):1243–1254. doi: 10.1261/rna.079674.123

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© 2023 Silveira d'Almeida et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — RNAi knockdown of RRP44. (A) Triplicate growth curve of T. brucei cells subjected to tetracycline-induced protein knockdown where total RNA was collected on day 4. (B) Northern blot hybridization performed on the extracted total RNA samples revealing the mature and alternate conformers of tRNA^Tyr and tRNA^Asp. (C,D) Relative amounts of alternate conformer tRNA^Tyr and tRNA^Asp, and mature tRNA^Tyr and tRNA^Asp. The graphs are the result of three biological replicas. The asterisks in C denote significance based on a two-tailed paired t-test with a P-value of 0.0009. The asterisks in D denote P-values of 0.0008 for both alt-tRNA^Asp and tRNA^Asp. “ns” stands for nonsignificant by the same test.